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Phosphotyrosine Antibody, Biotin
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Catalog# roduct Description |
ICP9901 The affinity purified rabbit anti-phospho-tyrosine antibody was conjugated to d-biotin via N-acetylation. The conjugates are useful for the detection, identification, and quantization of tyrosine-phosphorylated proteins and peptides. These conjugates have been utilized for non-radioactive tyrosine kinase assays. |
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Species
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Rabbit
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Formulation
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250 μg/mL in PBS, 50% glycerol, 0.01% NaN3
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Immunogen
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Iodoacetylphosphotyamine-KLH conjugates
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Purification
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The antibody was affinity purified with phospho-tyrosine immobilized on agarose.
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Specificity
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The antibody is pan-specific against phospho-tyrosine. There are no cross reactions with phospho-serine or phospho-threonine.
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Applications
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ELISA (0.25 - 1.0 μg/mL); WB (0.25 - 1.0 μg/mL); IP (5 μg/mg protein); IHC (10 μg/mL)
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Scientific Description
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Threonine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to form phospho-tyrosine, which is considered a key step in the regulation of enzymatic activities such as signal transduction.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is one year from date of shipping if properly stored.
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Product Specific References |
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